Russian cosmonaut builds engineered cartilage on board the International Space Station using new technique that could be used to treat injuries on future deep space missions
- Cosmonaut Oleg Kononenko carried out the experiment to ‘engineer cartilage’
- Researchers say the technique could be used on long-duration space flights
- It could be used to heal certain types of injuries or in bone growth in future
- Previous techniques required a type of scaffolding not practical in microgravity
Russian cosmonauts have built an engineered cartilage in the microgravity of the International Space Station for the first time, and it could help in long space flights.
Oleg Kononenko used a new type of ‘scaffold-free’ tissue engineering approach developed by Moscow firm 3D Bioprinting Solutions that uses magnetic fields.
The technique draws on the power of magnetic fields to overcome obstacles faced by traditional scaffolding-based approaches to cartilage engineering.
This approach, called ‘levitational bioassembly’, may also pave the way for advances in space regenerative medicine that could be used in long-distance space travel where astronauts and cosmonauts may be away from Earth for months or years.
Oleg Kononenko used a new type of ‘scaffold-free’ tissue engineering approach developed by Moscow firm 3D Bioprinting Solutions that uses magnetic fields
Tissue cells were placed in a temperature-controlled chamber to release the cartilage cells, then placed the cuvettes into the magnetic bioassembler to begin constructing tissue as seen in this image
Scientists have been pursuing scaffold-free engineering approaches that coax cells to self-assemble and develop into tissue without the help of a platform for years.
While magnetic levitational bioassembly has previously piqued researchers’ interest, prior techniques have relied on agents that are toxic to living cells in high levels.
To overcome this challenge lead author Vladislav Parfenov, from 3D Bioprinting Solutions, developed mathematical and computer models.
These were designed to find out if magnetic fields could be used for self-assembly of tissue in the microgravity of space.
Computer simulations were used to model how tissue spheroids, or 3D aggregates of cells in culture that retain the tissue’s architecture and functions, would fuse under microgravity conditions.
The researchers then developed viable tissue spheroids in a biological laboratory in Baikonur Cosmodrome in Khazakhstan using human cartilage cells.
The cells were embedded within hydrogel inside charged cuvettes and delivered to the Russian segment of the International Space Station along with a novel, custom-designed magnetic bioassembler.
While on board, a cosmonaut cooled the hydrogel down in a temperature-controlled chamber to release the cartilage cells, then placed the cuvettes into the magnetic bioassembler to begin constructing tissue.
This approach, called ‘levitational bioassembly’, may also pave the way for advances in space regenerative medicine that could be used in long-distance space travel where astronauts and cosmonauts may be away from Earth for months or years. They used a custom bioassembler
Oleg Kononenko unpacks the various parts of the experiment sent from Earth including the bioassembly device and human tissue cells preserved in hydrogel
Parfenov said the technique may also enable scientists to develop constructs in space that consist of both biological and inorganic materials.
This could include bone tissue equivalents for repairing limb damage on long flights.
‘The development of modern technologies for deep space exploration and the extension of manned space capabilities steadily increase the importance of space biotechnologies,’ said Parfenov.
It has already become possible to grow different species of plants producing oxygen and nutrients on the ISS, to obtain 3D biofilms of bacteria with altered synthetic and physiological activity, and to grow large protein crystals.
‘In this regard, the development of tissue engineering approaches to create complete equivalents of human tissues and organs to study the influence of space flight conditions and to meet the needs of space medicine is the next con- sequential step,’ he said.
The research has been published in the journal Science Advances.